Urease activity in the crystalline state
نویسندگان
چکیده
منابع مشابه
Studies on Crystalline Urease
1. Crystalline urease is not inactivated by trypsin in the absence of a gum. In fact, the presence of trypsin alone in aqueous solutions of urease has an action similar to that of gum, that is, it acts as a "protective colloid" for urease. 2. Crystalline urease is inactivated by trypsin in the presence of a gum. This occurs with great rapidity if purified (crystalline) trypsin is used. 3. If tr...
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A number of different enzymes are activated by certain reducing agents (e.g., KCN, H&S, cysteine, and reduced glutathione) and inactivated by oxidizing agents (e.g., K3Fe(CN)&, HzOz, and cystine). Under ideal conditions such inactivation is reversible by the addition of reducing agents (for a review of this subject cJ Hellerman (1937, 1939)). These results have been interpreted on the basis of ...
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1. The hydrolysis of urea catalyzed by jack bean meal has been followed by determining colorimetrically after Nesslerization the ammonia nitrogen, and volumetrically the carbon dioxide liberated at successive intervals during the reaction. During the early part of hydrolysis the rate of ammonia or carbon dioxide liberation is constant for all the urease solutions which were used. 2. When log ra...
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Many studies of the kinetics of enzyme action as a function of temperature have been made. The older work (for reference cf. Haldane, 1930; Tauber, 1937) indicates that while the velocity of an enzyme-catalyzed reaction increases with rise in temperature, up to the inactivation temperature of the enzyme, this change in rate is not an exponential function of the absolute temperature. I t has bee...
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The relationship postulated between hepatic coma and hyperammonaemia implicates the gastrointestinal tract as a major source of the excess ammonia in the peripheral blood of some patients with cirrhosis (Butt and Summerskill, 1961; Chalmers, 1960; Sherlock, Summerskill, White, and Phear, 1954). Conventional therapy, comprising protein restriction, antibiotics, and enemas, is directed toward eli...
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ژورنال
عنوان ژورنال: Protein Science
سال: 1995
ISSN: 0961-8368,1469-896X
DOI: 10.1002/pro.5560041028